Protein Splicing in Vitrowith a Semisynthetic Two-component Minimal Intein
نویسندگان
چکیده
منابع مشابه
The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein.
The 198-amino-acid in-frame insertion in the gyrA gene of Mycobacterium xenopi is the smallest known naturally occurring active protein splicing element (intein). Comparison with other mycobacterial gyrA inteins suggests that the M. xenopi intein underwent a complex series of events including (i) removal of 222 amino acids that encompass most of the central intein domain, and (ii) addition of a...
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Protein trans-splicing (PTS) by split inteins has found widespread use in chemical biology and biotechnology. Herein, we describe the use of a consensus design approach to engineer a split intein with enhanced stability and activity that make it more robust than any known PTS system. Using batch mutagenesis, we first conduct a detailed analysis of the difference in splicing rates between the Np...
متن کاملConstruction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation.
A new split intein-based protein ligation tool that is synthetically accessible and can be used for protein semisynthesis on the cell surface and potentially inside cells has been constructed.
متن کاملBranching out of the intein active site in protein splicing.
Inteins perform a macromolecular vanishing act that continues to impress as its secrets are revealed. Dispersed through all domains of life, these autocatalytic domains exit the folds of ostensibly unrelated proteins soon after translation. Inteins bust at the seams, severing peptide bonds that immediately precede and follow their sequence. Critically, they also sew up the damage; the two polyp...
متن کاملAn Engineered Split Intein for Photoactivated Protein Trans-Splicing
Protein splicing is mediated by inteins that auto-catalytically join two separated protein fragments with a peptide bond. Here we engineered a genetically encoded synthetic photoactivatable intein (named LOVInC), by using the light-sensitive LOV2 domain from Avena sativa as a switch to modulate the splicing activity of the split DnaE intein from Nostoc punctiforme. Periodic blue light illuminat...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.26.15887